Charles University's collection of ancient coins in its present state has existed since l945. Greek, Roman and other ancient coins were entrusted to the care of the Seminar for Ancient History and compiled into a single collection along with antique coins from the original collection of the Czech University. The collection contains about 4,000 pieces altogether, mostly in silver and bronze.This publication presents a catalogue of 564 Roman Imperial coins dating from 235 to 284 A. D. and is part of the efforts to gradually catalogue the entirety of Charles University's collection of ancient coins, stored in the depository of the CU Institute of Greek and Latin Studies, with the aim to acquaint the public with the vast ancient coin collection of Charles University. The publication builds upon Václav Marek's 1985 monograph The Roman Republican Coins in the Collection of Charles University and Federico Gambacorta's 2013 continuation titled Catalogue of the Late Roman, Byzantine and Barbaric Coins in the Charles University Collection (364-1092 A. D.).